Part of the Comprehensive Cancer Center, this core provides establishment of cell lines from primary murine and human tissue explants, production of virus stocks for gene transfer and large-scale expansion of tumor cell lines for downstream applications. The lab houses specialized equipment such as the Caliper IVIS100 In Vivo Imaging System, which increases the versatility of experimental techniques in this area of research.

This core laboratory provides access to X-ray crystallographic equipment, technical support, high-performance computing and scientific consultation with an emphasis on biological processes related to cancer such as cell signaling, transcriptional regulation, DNA damage and repair, lipid metabolism and redox regulation. The lab exists to meet the growing needs for structure determination and computational analysis of protein and DNA/RNA structure, function and dynamics for projects ranging from basic science to drug design.

This lab provides analysis and sorting of cells using up to nine fluorescent parameters. Examples of analyses available from the Flow Cytometry Lab include DNA content measurement, cell cycle specific nuclear and cytoplasmic proteins, apoptosis markers, stem cell populations, cell surface markers, cell viability and membrane potential. Cell lysates can also be analyzed with bead substrate based immunoassays.

Providing services in analytical and quantitative measurements of lipid and lipoprotein endpoints as well as in atherosclerosis evaluations in animal models, the Lipid, Lipoprotein and Atherosclerosis Analysis lab offers an array of analyses including major lipid class quantification, lipid class fatty acid analysis, plasma and tissue sterol analysis, dietary compositional analysis, plasma lipoprotein isolation and quantification and isolation of plasma lipoproteins, among others. These analyses are supported by capabilities in gas liquid chromatography, gel filtration chromatography, preparative ultracentrifugation, RNA extraction, cDNA synthesis and quantitative PCR.

This core laboratory offers biophysical insights into biomedical, pharmaceutical and biotechnological research through capabilities and expertise in biological macromolecules. Through Molecular Partners, you can access biotechnologies including surface plasmon resonance, UV/visible spectroscopy, fluorescence spectroscopy, circular dichroic spectroscopy, light scattering and analytical ultracentrifugation.

This core provides access to, technical support of and data processing for the Vevo LAZR Photoacoustic imaging system for studies of preclinical rodent models. This technology platform provides non-invasive functional, anatomic and visual information, including never-before-seen insights into cellular environment and flow of blood.

Capitalize on the state-of-the-art imaging modalities at Wake Forest Baptist through the Translational Imaging Program. Specialized imaging modalities include a Siemens MAGNETOM Skyra 3T MRI Scanner, a Toshiba 32-slice Aquilion CT Scanner dedicated to animal imaging, a GE PETtrace 10 Cyclotron and a GE 16-slice PET/CT Discovery ST Scanner. Technologists provide support on all of these imaging modalities, as well as assistance with imaging informatics. Studies can make use of a range of imaging facilities, including an MRI research facility, two organic chemistry labs, metabolite analysis lab and radiochemistry lab.

Access the expertise and technology of the Virtual Microscopy Core to generate detailed virtual images of glass slides by using state-of-the-art digital technology. The experts of the Virtual Microscopy Core use the Olympus VS-110 Virtual Imaging System and a Hammamatsu NanoZoomer to create micrographs that allow 3D viewing and manipulation. The lab’s capabilities enable researchers to perform measurements and quantitative analysis more efficiently and to capture images with the clarity.